Hotspot

trnK-UUU-rps16

LSC · 4062-4651 · 590 bp

Back to Nothoscordum

Pi 0.00509

MarkerSeek score 52.20

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 590
variable_sites: 3
pi: 0.00509
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	AAAGCCGAGTACTCTACCGT	GGTGCTCAACCTACAGGAAC	50.0	55.0	58.2	58.2	696-698 bp	1.0	77.6
trnK-UUU-rps16_p2	AAGCCGAGTACTCTACCGTT	GGTGCTCAACCTACAGGAAC	50.0	55.0	58.2	58.2	695-697 bp	1.0	77.6
trnK-UUU-rps16_p3	TACCGTTGAGTTAGCAACCC	GGTGCTCAACCTACAGGAAC	50.0	55.0	57.5	58.2	682-684 bp	1.0	75.3
trnK-UUU-rps16_p4	AGTCGCACTTAAAAGCCGAG	GGTGCTCAACCTACAGGAAC	50.0	55.0	58.6	58.2	707-709 bp	1.0	74.4
trnK-UUU-rps16_p5	TCGCACTTAAAAGCCGAGTA	GGTGCTCAACCTACAGGAAC	45.0	55.0	57.3	58.2	705-707 bp	1.0	73.1