Hotspot

matK-trnK-UUU

LSC · 3246-4024 · 779 bp

Back to Nothoscordum

Pi 0.00394

MarkerSeek score 63.20

Primer available yes

Label matK-trnK-UUU

Feature Metadata

label_name: matK-trnK-UUU
feature_type: igs
region: LSC
length_bp: 779
variable_sites: 3
pi: 0.00394
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 63.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-trnK-UUU_p1	GTGTTGTTGCCGGGATCTAT	TCTGGGTTGCTAACTCAACG	50.0	50.0	58.0	57.8	852-870 bp	1.0	80.3
matK-trnK-UUU_p2	GTGTTGTTGCCGGGATCTAT	AACGGTAGAGTACTCGGCTT	50.0	50.0	58.0	58.2	836-854 bp	1.0	80.3
matK-trnK-UUU_p3	GTGTTGTTGCCGGGATCTAT	ACGGTAGAGTACTCGGCTTT	50.0	50.0	58.0	58.2	835-853 bp	1.0	80.3
matK-trnK-UUU_p4	ATAGGAAGTGTTGTTGCCGG	TCTGGGTTGCTAACTCAACG	50.0	50.0	58.2	57.8	859-877 bp	1.0	79.2
matK-trnK-UUU_p5	ATAGGAAGTGTTGTTGCCGG	ACGGTAGAGTACTCGGCTTT	50.0	50.0	58.2	58.2	842-860 bp	1.0	79.2