Hotspot

rps8

LSC · 78372-78770 · 399 bp

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Pi 0.00251

MarkerSeek score 28.50

Primer available yes

Label rps8

Feature Metadata

label_name: rps8
feature_type: gene
region: LSC
length_bp: 399
variable_sites: 2
pi: 0.00251
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 28.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps8_p1	CGTTGGGAAGCGATTCAGTA	GCGGATAATAGTGGAGCTCG	50.0	55.0	58.0	58.0	1125 bp	1.0	80.9
rps8_p2	CGTTGGGAAGCGATTCAGTA	CGAGTCCTAGGAGCTGGTAA	50.0	55.0	58.0	57.9	1089 bp	1.0	80.6
rps8_p3	CAGGAGGAAGGGGTGTAAGA	GCGGATAATAGTGGAGCTCG	55.0	55.0	58.0	58.0	964 bp	1.0	80.6
rps8_p4	CGTTGGGAAGCGATTCAGTA	GAACTCGAGTTTTTGGTGCG	50.0	50.0	58.0	58.0	872 bp	0.75	70.2
rps8_p5	CAGGAGGAAGGGGTGTAAGA	GAACTCGAGTTTTTGGTGCG	55.0	50.0	58.0	58.0	711 bp	0.75	69.9