Hotspot

rps19

IRb · 82300-82578 · 279 bp

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Pi 0.00538

MarkerSeek score 31.90

Primer available yes

Label rps19

Feature Metadata

label_name: rps19
feature_type: gene
region: IRb
length_bp: 279
variable_sites: 3
pi: 0.00538
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 31.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps19_p1	GCTTCTTCATAGGAGCGTCC	TGGGTAGAGCCGGATCTAAG	55.0	55.0	58.1	58.0	1001-1041 bp	1.0	83.5
rps19_p2	CATCTTCCTGTATACCCGCG	GTTATCCTGCGCTTGGAAGA	55.0	50.0	57.9	58.0	1288-1328 bp	1.0	83.0
rps19_p3	CATCTTCCTGTATACCCGCG	TGGGTAGAGCCGGATCTAAG	55.0	55.0	57.9	58.0	1426-1466 bp	1.0	82.9
rps19_p4	GAGCCCGGGGTCTTAATTTT	TGGGTAGAGCCGGATCTAAG	50.0	55.0	57.9	58.0	1174-1214 bp	1.0	82.9
rps19_p5	CATCTTCCTGTATACCCGCG	GAGGGTAAATCGGCCACATT	55.0	50.0	57.9	57.9	1538-1578 bp	1.0	82.2