Hotspot

rpl16

LSC · 79475-80882 · 1408 bp

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Pi 0.00391

MarkerSeek score 40.90

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1408
variable_sites: 11
pi: 0.00391
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	CGAGCTCCACTATTATCCGC	CGCGGGTATACAGGAAGATG	55.0	55.0	58.0	57.9	2315-2335 bp	1.0	80.3
rpl16_p2	CGCACCAAAAACTCGAGTTC	CGCGGGTATACAGGAAGATG	50.0	55.0	58.0	57.9	2568-2588 bp	1.0	80.3
rpl16_p3	CGAGCTCCACTATTATCCGC	GCCGTTCTGTCCAGTGATAG	55.0	55.0	58.0	58.1	1729-1749 bp	1.0	80.3
rpl16_p4	CGCACCAAAAACTCGAGTTC	GCCGTTCTGTCCAGTGATAG	50.0	55.0	58.0	58.1	1982-2002 bp	1.0	80.2
rpl16_p5	TTACCAGCTCCTAGGACTCG	CGCGGGTATACAGGAAGATG	55.0	55.0	57.9	57.9	2351-2371 bp	1.0	80.0