Hotspot

psaB

LSC · 35753-37957 · 2205 bp

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Pi 0.000907

MarkerSeek score 57.90

Primer available yes

Label psaB

Feature Metadata

label_name: psaB
feature_type: gene
region: LSC
length_bp: 2205
variable_sites: 4
pi: 0.000907
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaB_p1	CCCTTCTCCATCGAAACCAA	TACAAGGACGTGCTGTAGGA	50.0	50.0	57.5	58.1	2388 bp	1.0	75.5
psaB_p2	CCCTTCTCCATCGAAACCAA	TTACCTTCTGGGCGGAATTG	50.0	50.0	57.5	57.9	2360 bp	1.0	75.2
psaB_p3	GGATCCCCTTCTCCATCGAA	TACAAGGACGTGCTGTAGGA	55.0	50.0	58.6	58.1	2393 bp	1.0	74.8
psaB_p4	GGATCCCCTTCTCCATCGAA	TTACCTTCTGGGCGGAATTG	55.0	50.0	58.6	57.9	2365 bp	1.0	74.6
psaB_p5	CCCTTCTCCATCGAAACCAA	AGGACGTGCTGTAGGAGTAA	50.0	50.0	57.5	57.8	2384 bp	1.0	74.5