Hotspot

ndhG-ndhI

SSC · 117071-117472 · 402 bp

Back to Notholirion

Pi 0.0266

MarkerSeek score 73.90

Primer available yes

Label ndhG-ndhI

Feature Metadata

label_name: ndhG-ndhI
feature_type: igs
region: SSC
length_bp: 402
variable_sites: 21
pi: 0.0266
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhI_p1	TCCCAGGCCCCCAAATATAA	GTCGGTTACCAATGTCAGTA	50.0	45.0	58.1	54.8	590-594 bp	1.0	57.3
ndhG-ndhI_p2	TCCCAGGCCCCCAAATATAA	GTCGGTTACCAATGTCAGTAA	50.0	42.9	58.1	55.6	590-594 bp	1.0	55.8
ndhG-ndhI_p3	TCCCAACGAAAAGGCAGAAT	GTCGGTTACCAATGTCAGTA	45.0	45.0	57.7	54.8	635-639 bp	1.0	55.4
ndhG-ndhI_p4	CCCAGGCCCCCAAATATAAG	GTCGGTTACCAATGTCAGTA	55.0	45.0	57.7	54.8	589-593 bp	1.0	55.4
ndhG-ndhI_p5	TAATACTACTCCCAGGCCCC	GTCGGTTACCAATGTCAGTA	55.0	45.0	57.6	54.8	599-603 bp	1.0	54.6