Hotspot

rpl22-rps19

LSC · 82078-82299 · 222 bp

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Pi 0.0417

MarkerSeek score 71.00

Primer available yes

Label rpl22-rps19

Feature Metadata

label_name: rpl22-rps19
feature_type: igs
region: LSC
length_bp: 222
variable_sites: 17
pi: 0.0417
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 71.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22-rps19_p1	GCTTCTTCATAGGAGCGTCC	GTTATCCTGCGCTTGGAAGA	55.0	50.0	58.1	58.0	863-903 bp	1.0	84.1
rpl22-rps19_p2	GAGCCCGGGGTCTTAATTTT	GTTATCCTGCGCTTGGAAGA	50.0	50.0	57.9	58.0	1036-1076 bp	1.0	83.3
rpl22-rps19_p3	CATCTTCCTGTATACCCGCG	GTTATCCTGCGCTTGGAAGA	55.0	50.0	57.9	58.0	1288-1328 bp	1.0	83.0
rpl22-rps19_p4	TTCGAAACCTGAGGACGTTC	GTTATCCTGCGCTTGGAAGA	50.0	50.0	57.9	58.0	1353-1393 bp	1.0	82.2
rpl22-rps19_p5	CTTCGAAACCTGAGGACGTT	GTTATCCTGCGCTTGGAAGA	50.0	50.0	57.8	58.0	1354-1394 bp	1.0	82.2