Hotspot

rpl20-rps12

LSC · 67080-67851 · 772 bp

Back to Notholirion

Pi 0.00345

MarkerSeek score 61.10

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 772
variable_sites: 5
pi: 0.00345
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	GTCTCCGAGCTGTATACCCT	AAATGTCACGAAATCCCCCG	55.0	50.0	58.0	58.5	876 bp	1.0	76.0
rpl20-rps12_p2	AGTCTTGAATGAGCCCCTCT	AAATGTCACGAAATCCCCCG	50.0	50.0	58.0	58.5	931 bp	1.0	75.8
rpl20-rps12_p3	TGAGCCCCTCTAAAGGTTGA	AAATGTCACGAAATCCCCCG	50.0	50.0	58.0	58.5	922 bp	1.0	75.8
rpl20-rps12_p4	GTCTCCGAGCTGTATACCCT	CAGCGTCGAGGAACATGTAC	55.0	55.0	58.0	58.7	839 bp	1.0	74.5
rpl20-rps12_p5	TTTTTGTTCGACGTCTCCGA	AAATGTCACGAAATCCCCCG	45.0	50.0	57.8	58.5	888 bp	1.0	74.5