Hotspot

petA-psbJ

LSC · 61222-62032 · 811 bp

Back to Notholirion

Pi 0.00594

MarkerSeek score 56.20

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 811
variable_sites: 8
pi: 0.00594
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.86
markerseek_score: 56.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GGCATCTGTTATTTTGGCACA	GGTTCATATTCCGGGTTGGG	42.9	55.0	57.4	58.3	819-929 bp	1.0	69.5
petA-psbJ_p2	TGGCATCTGTTATTTTGGCAC	GGTTCATATTCCGGGTTGGG	42.9	55.0	57.4	58.3	820-930 bp	1.0	69.5
petA-psbJ_p3	GGCATCTGTTATTTTGGCACA	CATATTCCGGGTTGGGTTCA	42.9	50.0	57.4	57.6	815-925 bp	1.0	68.6
petA-psbJ_p4	TGGCATCTGTTATTTTGGCAC	CATATTCCGGGTTGGGTTCA	42.9	50.0	57.4	57.6	816-926 bp	1.0	68.6
petA-psbJ_p5	GGCATCTGTTATTTTGGCACA	GTTCATATTCCGGGTTGGGT	42.9	50.0	57.4	57.6	818-928 bp	1.0	68.6