Hotspot

ycf4-cemA

LSC · 58602-59545 · 944 bp

Back to Notholirion

Pi 0.00812

MarkerSeek score 56.40

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 944
variable_sites: 15
pi: 0.00812
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	AAAAGCTGCCGAATTAGCCT	ACCGTTTCCTTGTGTATTCCT	45.0	42.9	58.2	57.2	1061-1067 bp	1.0	65.8
ycf4-cemA_p2	CCTTGACTGGCACTGATGAG	ACCGTTTCCTTGTGTATTCCT	55.0	42.9	58.3	57.2	1107-1113 bp	1.0	64.9
ycf4-cemA_p3	AAAAAGCTGCCGAATTAGCC	ACCGTTTCCTTGTGTATTCCT	45.0	42.9	57.3	57.2	1062-1068 bp	1.0	61.9
ycf4-cemA_p4	AAAGCTGCCGAATTAGCCTA	ACCGTTTCCTTGTGTATTCCT	45.0	42.9	57.3	57.2	1060-1066 bp	1.0	61.4
ycf4-cemA_p5	AAAAGCTGCCGAATTAGCCT	ACCGTTTCCTTGTGTATTCC	45.0	45.0	58.2	55.7	1061-1067 bp	1.0	61.4