Hotspot

accD-psaI

LSC · 56785-57582 · 798 bp

Back to Notholirion

Pi 0.01

MarkerSeek score 56.50

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 798
variable_sites: 16
pi: 0.01
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	TCAGCTCCACGGTTTCTTTC	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.1	58.0	908-928 bp	1.0	80.3
accD-psaI_p2	AAGGGGTGTTCTGAGTGAGT	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.2	58.0	932-952 bp	1.0	79.7
accD-psaI_p3	AGGGGTGTTCTGAGTGAGTT	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.2	58.0	931-951 bp	1.0	79.7
accD-psaI_p4	TCAGCTCCACGGTTTCTTTC	AAGAAGCCATTGCAATTGCC	50.0	45.0	58.1	57.9	914-934 bp	1.0	79.7
accD-psaI_p5	AGGGGTGTTCTGAGTGAGTT	AAGAAGCCATTGCAATTGCC	50.0	45.0	58.2	57.9	937-957 bp	1.0	79.1