Hotspot

psaA-ycf3

LSC · 40236-40824 · 589 bp

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Pi 0.00877

MarkerSeek score 55.80

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 589
variable_sites: 10
pi: 0.00877
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	TGGCCCATTCCTCAAAAGAC	TTGAAGATCACGAGGCGTTT	50.0	45.0	58.1	57.8	701-708 bp	1.0	79.6
psaA-ycf3_p2	GTCTGGCCCATTCCTCAAAA	TTGAAGATCACGAGGCGTTT	50.0	45.0	58.1	57.8	704-711 bp	1.0	79.5
psaA-ycf3_p3	TGTTCTCGAGAAATGGCCTG	TTGAAGATCACGAGGCGTTT	50.0	45.0	57.9	57.8	724-731 bp	1.0	79.3
psaA-ycf3_p4	GTCTGGCCCATTCCTCAAAA	TTGGTTGAAGATCACGAGGC	50.0	50.0	58.1	58.2	708-715 bp	1.0	79.3
psaA-ycf3_p5	TGGCCCATTCCTCAAAAGAC	TTGGTTGAAGATCACGAGGC	50.0	50.0	58.1	58.2	705-712 bp	1.0	79.3