Hotspot

psbM-trnD-GUC

LSC · 27898-29079 · 1182 bp

Back to Notholirion

Pi 0.00513

MarkerSeek score 55.90

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 1182
variable_sites: 12
pi: 0.00513
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 55.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	GCGGTAGGAACTAGAATGAACA	CCTCGCTGGGATTGTAGTTC	45.5	55.0	57.9	58.1	1318-1334 bp	1.0	64.1
psbM-trnD-GUC_p2	GCGGTAGGAACTAGAATGAACA	AGTTCAATCGGTCAGAGCAC	45.5	50.0	57.9	57.9	1303-1319 bp	1.0	63.9
psbM-trnD-GUC_p3	TTGACTGGCTGTTTTTACGT	CCTCGCTGGGATTGTAGTTC	40.0	55.0	55.8	58.1	1355-1371 bp	1.0	63.5
psbM-trnD-GUC_p4	GCGGTAGGAACTAGAATGAACA	TACACCTCGCTGGGATTGTA	45.5	50.0	57.9	58.1	1322-1338 bp	1.0	63.4
psbM-trnD-GUC_p5	TTGACTGGCTGTTTTTACGT	AGTTCAATCGGTCAGAGCAC	40.0	50.0	55.8	57.9	1340-1356 bp	1.0	63.3