Hotspot

trnK-UUU-rps16

LSC · 4055-4786 · 732 bp

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Pi 0.0146
MarkerSeek score 59.80
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
732
variable_sites
21
pi
0.0146
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.99
markerseek_score
59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AAAGCCGAGTACTCTACCGT AGGAAGACAGAATTATTTAAAGAAGGA 50.0 29.6 58.2 56.7 785-805 bp 1.0 44.1
trnK-UUU-rps16_p2 AAGCCGAGTACTCTACCGTT AGGAAGACAGAATTATTTAAAGAAGGA 50.0 29.6 58.2 56.7 784-804 bp 1.0 44.1
trnK-UUU-rps16_p3 AAAGCCGAGTACTCTACCGT GGAAGACAGAATTATTTAAAGAAGGA 50.0 30.8 58.2 55.5 784-804 bp 1.0 44.1
trnK-UUU-rps16_p4 AAGCCGAGTACTCTACCGTT GGAAGACAGAATTATTTAAAGAAGGA 50.0 30.8 58.2 55.5 783-803 bp 1.0 44.1
trnK-UUU-rps16_p5 AAGCCGAGTACTCTACCGTT AGGAAGACAGAATTATTTAAAGAAGG 50.0 30.8 58.2 55.5 784-804 bp 1.0 44.1