Hotspot

ycf4

LSC · 61708-62262 · 555 bp

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Pi 0.0024

MarkerSeek score 59.30

Primer available yes

Label ycf4

Feature Metadata

label_name: ycf4
feature_type: gene
region: LSC
length_bp: 555
variable_sites: 2
pi: 0.0024
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4_p1	TGGGGTATTCTCTCAATTCCA	TTCTTGCATTTTGCCCTCCT	42.9	45.0	56.0	58.0	658 bp	1.0	56.3
ycf4_p2	TGGGGTATTCTCTCAATTCCAA	TTCTTGCATTTTGCCCTCCT	40.9	45.0	56.8	58.0	658 bp	1.0	54.4
ycf4_p3	TTGGGGTATTCTCTCAATTCCA	TTCTTGCATTTTGCCCTCCT	40.9	45.0	56.8	58.0	659 bp	1.0	54.4
ycf4_p4	AGTTGATGAGAGTTACTTCGGA	TTCTTGCATTTTGCCCTCCT	40.9	45.0	56.6	58.0	708-709 bp	1.0	53.0
ycf4_p5	TGATGAGAGTTACTTCGGAAACA	TTCTTGCATTTTGCCCTCCT	39.1	45.0	57.5	58.0	705-706 bp	1.0	52.5