Hotspot

rpoC1

LSC · 21584-24416 · 2833 bp

Back to Notelaea

Pi 0.000235

MarkerSeek score 56.20

Primer available yes

Label rpoC1

Feature Metadata

label_name: rpoC1
feature_type: gene
region: LSC
length_bp: 2833
variable_sites: 1
pi: 0.000235
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC1_p1	CGGAACTATGGGCCTAGTTG	TTCGATTGCTCGTTCGAGAA	55.0	45.0	57.8	57.7	2995-2997 bp	1.0	75.4
rpoC1_p2	CGGAACTATGGGCCTAGTTG	TTTCGATTGCTCGTTCGAGA	55.0	45.0	57.8	57.7	2996-2998 bp	1.0	75.4
rpoC1_p3	ATTCCGGAACTATGGGCCTA	TCGATTGCTCGTTCGAGAAT	50.0	45.0	57.9	57.4	2998-3000 bp	1.0	74.7
rpoC1_p4	CGGAACTATGGGCCTAGTTG	TCGATTGCTCGTTCGAGAAT	55.0	45.0	57.8	57.4	2994-2996 bp	1.0	73.7
rpoC1_p5	TTCCGGAACTATGGGCCTAG	TTCGATTGCTCGTTCGAGAA	55.0	45.0	58.6	57.7	2998-3000 bp	1.0	72.7