Hotspot

clpP

LSC · 72104-74143 · 2040 bp

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Pi 0.000654

MarkerSeek score 58.10

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 2040
variable_sites: 2
pi: 0.000654
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	TGGGCTGGTTTAGATTGATCC	TGTTGTTACGTTTCCACATCA	47.6	38.1	57.7	56.0	2214-2216 bp	1.0	46.1
clpP_p2	TGGGCTGGTTTAGATTGATCC	TGTTGTTACGTTTCCACATCAA	47.6	36.4	57.7	56.8	2214-2216 bp	1.0	43.9
clpP_p3	GGGCTGGTTTAGATTGATCCT	TGTTGTTACGTTTCCACATCA	47.6	38.1	57.4	56.0	2213-2215 bp	1.0	43.8
clpP_p4	TGGGCTGGTTTAGATTGATCC	ATGTTGTTACGTTTCCACATCA	47.6	36.4	57.7	56.6	2215-2217 bp	1.0	42.2
clpP_p5	TGGGCTGGTTTAGATTGATCC	TGTTACGTTTCCACATCAAAGT	47.6	36.4	57.7	56.5	2211-2213 bp	1.0	41.7