Hotspot

accD-psaI

LSC · 60453-61153 · 701 bp

Back to Notelaea

Pi 0.00285

MarkerSeek score 60.60

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 701
variable_sites: 3
pi: 0.00285
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	GCTCCACGCTTTCTTTCCTT	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.8	58.0	799 bp	1.0	74.0
accD-psaI_p2	GCTCCACGCTTTCTTTCCTT	AAGAAGCCATTGCAATTGCC	50.0	45.0	58.8	57.9	805 bp	1.0	73.4
accD-psaI_p3	GCTCCACGCTTTCTTTCCT	CCATTGCAATTGCCGGAAAT	52.6	45.0	58.1	58.0	799 bp	1.0	71.6
accD-psaI_p4	TCCACGCTTTCTTTCCTTTGA	CCATTGCAATTGCCGGAAAT	42.9	45.0	58.1	58.0	797 bp	1.0	71.5
accD-psaI_p5	GCTCCACGCTTTCTTTCCT	AAGAAGCCATTGCAATTGCC	52.6	45.0	58.1	57.9	805 bp	1.0	70.9