Hotspot

trnM(CAU)-psbD

LSC · 32955-34270 · 1316 bp

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Pi 0.00203
MarkerSeek score 60.10
Primer available yes
Label trnM(CAU)-psbD

Feature Metadata

label_name
trnM(CAU)-psbD
feature_type
igs
region
LSC
length_bp
1316
variable_sites
4
pi
0.00203
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnM(CAU)-psbD_p1 GTGGTAGAGTAACGCCATGG GGACCAGCCTACAAAAACGA 55.0 50.0 58.1 58.1 1475-1476 bp 1.0 78.9
trnM(CAU)-psbD_p2 TCAGTGGTAGAGTAACGCCA GGACCAGCCTACAAAAACGA 50.0 50.0 58.1 58.1 1478-1479 bp 1.0 78.7
trnM(CAU)-psbD_p3 GTGGTAGAGTAACGCCATGG ACAAAAACGAAACGGTCCCT 55.0 45.0 58.1 58.2 1465-1466 bp 1.0 77.8
trnM(CAU)-psbD_p4 TCAGTGGTAGAGTAACGCCA ACAAAAACGAAACGGTCCCT 50.0 45.0 58.1 58.2 1468-1469 bp 1.0 77.6
trnM(CAU)-psbD_p5 GGCGTAAGTCATCGGTTCAA GGACCAGCCTACAAAAACGA 50.0 50.0 58.3 58.1 1452-1453 bp 1.0 77.1