Hotspot

trnS(GCU)-trnG(GCC)

LSC · 9039-9742 · 704 bp

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Pi 0.00191
MarkerSeek score 60.90
Primer available yes
Label trnS(GCU)-trnG(GCC)

Feature Metadata

label_name
trnS(GCU)-trnG(GCC)
feature_type
igs
region
LSC
length_bp
704
variable_sites
2
pi
0.00191
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
60.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS(GCU)-trnG(GCC)_p1 CAATCCGACGCTTTAGTCCA AGACCGAAAGACCCTTTAACT 50.0 42.9 58.0 56.6 804-811 bp 1.0 61.5
trnS(GCU)-trnG(GCC)_p2 AATCCGACGCTTTAGTCCAC AGACCGAAAGACCCTTTAACT 50.0 42.9 58.0 56.6 803-810 bp 1.0 61.5
trnS(GCU)-trnG(GCC)_p3 ATCAACGGAACCGGAAAGAG AGACCGAAAGACCCTTTAACT 50.0 42.9 57.9 56.6 868-875 bp 1.0 60.7
trnS(GCU)-trnG(GCC)_p4 CATCAACGGAACCGGAAAGA AGACCGAAAGACCCTTTAACT 50.0 42.9 58.2 56.6 869-876 bp 1.0 59.9
trnS(GCU)-trnG(GCC)_p5 TCATCAACGGAACCGGAAAG AGACCGAAAGACCCTTTAACT 50.0 42.9 58.2 56.6 870-877 bp 1.0 59.9