Hotspot

ycf1

SSC · 125364-130985 · 5622 bp

Back to Noronhia

Pi 0.00496

MarkerSeek score 46.20

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5622
variable_sites: 66
pi: 0.00496
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TTTTGAATGCTGACCCCCTC	CTATACGTGCTCAAAGGCGT	50.0	50.0	58.1	58.1	171 bp	1.0	82.0
ycf1_p2	TTTTGAATGCTGACCCCCTC	TACGTGCTCAAAGGCGTAAA	50.0	45.0	58.1	57.8	168 bp	1.0	81.5
ycf1_p3	TTTTGAATGCTGACCCCCTC	TCTATACGTGCTCAAAGGCG	50.0	50.0	58.1	57.8	172 bp	1.0	81.0
ycf1_p4	TTTTGAATGCTGACCCCCTC	ATACGTGCTCAAAGGCGTAA	50.0	45.0	58.1	57.6	169 bp	1.0	79.7
ycf1_p5	TTTTGAATGCTGACCCCCTC	CGTGCTCAAAGGCGTAAAAT	50.0	45.0	58.1	57.4	166 bp	1.0	77.9