Hotspot

matK

LSC · 2169-3689 · 1521 bp

Back to Noronhia

Pi 0.0046

MarkerSeek score 62.20

Primer available yes

Label matK

Feature Metadata

label_name: matK
feature_type: gene
region: LSC
length_bp: 1521
variable_sites: 17
pi: 0.0046
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK_p1	ACATCAACATTTCAGAATAGTGGA	CCTTGTTTTGACTGTATCGCA	33.3	42.9	56.4	56.8	1700 bp	1.0	41.5
matK_p2	ACATCAACATTTCAGAATAGTGGA	AGTTTACCTGTTTCCGAGGT	33.3	45.0	56.4	56.4	1739 bp	1.0	41.4
matK_p3	ACATCAACATTTCAGAATAGTGGA	AAGTTTACCTGTTTCCGAGGT	33.3	42.9	56.4	57.1	1740 bp	1.0	41.4
matK_p4	ACATCAACATTTCAGAATAGTGGAA	AGTTTACCTGTTTCCGAGGT	32.0	45.0	57.0	56.4	1739 bp	1.0	41.4
matK_p5	ACATCAACATTTCAGAATAGTGG	AGTTTACCTGTTTCCGAGGT	34.8	45.0	55.0	56.4	1739 bp	1.0	41.4