Hotspot

rpl32-trnL(UAG)

SSC · 114923-115780 · 858 bp

Back to Noronhia

Pi 0.00583

MarkerSeek score 59.50

Primer available yes

Label rpl32-trnL(UAG)

Feature Metadata

label_name: rpl32-trnL(UAG)
feature_type: igs
region: SSC
length_bp: 858
variable_sites: 11
pi: 0.00583
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL(UAG)_p1	TGGGACAGCGTTAAAAGCAT	TCCTAAGAGCAGCGTGTCTA	45.0	50.0	58.1	57.9	978-991 bp	1.0	79.6
rpl32-trnL(UAG)_p2	TGGGACAGCGTTAAAAGCAT	AGCAGCGTGTCTACCAATTT	45.0	45.0	58.1	57.8	971-984 bp	1.0	79.1
rpl32-trnL(UAG)_p3	TGGGACAGCGTTAAAAGCAT	CTAAGAGCAGCGTGTCTACC	45.0	55.0	58.1	57.8	976-989 bp	1.0	79.1
rpl32-trnL(UAG)_p4	TGGGACAGCGTTAAAAGCAT	CCTAAGAGCAGCGTGTCTAC	45.0	55.0	58.1	57.8	977-990 bp	1.0	79.0
rpl32-trnL(UAG)_p5	TGGGACAGCGTTAAAAGCAT	GAGCAGCGTGTCTACCAATT	45.0	50.0	58.1	58.3	972-985 bp	1.0	78.4