Hotspot

atpB-rbcL

LSC · 56492-57276 · 785 bp

Back to Noronhia

Pi 0.0107

MarkerSeek score 45.80

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 785
variable_sites: 21
pi: 0.0107
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	ATACGGCCCAGGTTTTGTTT	GTAAGTCCATCGGTCCACAC	45.0	55.0	58.0	58.0	1077-1078 bp	1.0	81.6
atpB-rbcL_p2	ATACGGCCCAGGTTTTGTTT	GGTAGCATCGCCCTTTGTAA	45.0	50.0	58.0	58.0	1109-1110 bp	1.0	81.3
atpB-rbcL_p3	ATACGGCCCAGGTTTTGTTT	AGAAACGATCTCTCCAACGC	45.0	50.0	58.0	58.0	1508-1509 bp	1.0	81.2
atpB-rbcL_p4	ATACGGCCCAGGTTTTGTTT	GGGGACGACCATACTTGTTC	45.0	55.0	58.0	58.0	1358-1359 bp	1.0	81.2
atpB-rbcL_p5	ATACGGCCCAGGTTTTGTTT	GTACCCGCAGTAGCATTCAA	45.0	50.0	58.0	58.0	1590-1591 bp	1.0	81.0