Hotspot

ndhC-trnM(CAU)

LSC · 52377-54318 · 1942 bp

Back to Noronhia

Pi 0.00505

MarkerSeek score 61.70

Primer available yes

Label ndhC-trnM(CAU)

Feature Metadata

label_name: ndhC-trnM(CAU)
feature_type: igs
region: LSC
length_bp: 1942
variable_sites: 22
pi: 0.00505
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnM(CAU)_p1	GGGGCTAAAATCCCGGAAAT	CCCGCCGTATGAAAGCAATA	50.0	50.0	57.9	58.4	2083-2087 bp	1.0	77.0
ndhC-trnM(CAU)_p2	GGGGCTAAAATCCCGGAAAT	AGGTACCATTGACTCCGGTA	50.0	50.0	57.9	57.5	2144-2148 bp	1.0	76.0
ndhC-trnM(CAU)_p3	GGGGCTAAAATCCCGGAAAT	TAGGTACCATTGACTCCGGT	50.0	50.0	57.9	57.5	2145-2149 bp	1.0	76.0
ndhC-trnM(CAU)_p4	GGGGCTAAAATCCCGGAAAT	CCGCCGTATGAAAGCAATAC	50.0	50.0	57.9	57.3	2082-2086 bp	1.0	75.0
ndhC-trnM(CAU)_p5	TTCTCCGGCCCTTTCCTAAT	CCCGCCGTATGAAAGCAATA	50.0	50.0	58.4	58.4	2104-2108 bp	1.0	74.4