Hotspot

ycf3-trnS(GGA)

LSC · 46186-46972 · 787 bp

Back to Noronhia

Pi 0.00953

MarkerSeek score 51.80

Primer available yes

Label ycf3-trnS(GGA)

Feature Metadata

label_name: ycf3-trnS(GGA)
feature_type: igs
region: LSC
length_bp: 787
variable_sites: 16
pi: 0.00953
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS(GGA)_p1	ACTTCACCTTCAACAAGCGT	AAGGGTTCGGGCAATTTTCT	45.0	45.0	58.0	58.0	1400-1407 bp	1.0	81.2
ycf3-trnS(GGA)_p2	ACTTCACCTTCAACAAGCGT	AGGGTTCGGGCAATTTTCTT	45.0	45.0	58.0	58.0	1399-1406 bp	1.0	81.2
ycf3-trnS(GGA)_p3	ACTTCACCTTCAACAAGCGT	CTCTCGACTCATCCCCTCAT	45.0	55.0	58.0	58.0	1577-1587 bp	1.0	81.0
ycf3-trnS(GGA)_p4	ACTTCACCTTCAACAAGCGT	AACCATTTGACCCTTCACCC	45.0	50.0	58.0	58.0	1545-1555 bp	1.0	81.0
ycf3-trnS(GGA)_p5	ACTTCACCTTCAACAAGCGT	TTCGATTGGGTATGGCTTCG	45.0	50.0	58.0	58.1	1742-1752 bp	1.0	80.6