Hotspot

trnS(UGA)-psbZ

LSC · 37294-37645 · 352 bp

Back to Noronhia

Pi 0.00966

MarkerSeek score 48.20

Primer available yes

Label trnS(UGA)-psbZ

Feature Metadata

label_name: trnS(UGA)-psbZ
feature_type: igs
region: LSC
length_bp: 352
variable_sites: 8
pi: 0.00966
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS(UGA)-psbZ_p1	CAACCACTCAGCCATCTCTC	AATCGAACCCGCATCTTCTC	55.0	50.0	58.0	58.1	904-913 bp	1.0	81.4
trnS(UGA)-psbZ_p2	CAACCACTCAGCCATCTCTC	GAATCGAACCCGCATCTTCT	55.0	50.0	58.0	58.1	905-914 bp	1.0	81.4
trnS(UGA)-psbZ_p3	CAACCACTCAGCCATCTCTC	AAGAAGGGATAGACTCGCGA	55.0	50.0	58.0	57.9	1005-1014 bp	1.0	81.2
trnS(UGA)-psbZ_p4	ATACCAATCCGAGCCAAACC	AAGAAGGGATAGACTCGCGA	50.0	50.0	57.9	57.9	1129-1138 bp	1.0	80.9
trnS(UGA)-psbZ_p5	ATCTAATGCGTTCCCCAACC	AAGAAGGGATAGACTCGCGA	50.0	50.0	57.9	57.9	1717-1730 bp	1.0	80.6