Hotspot

petN-psbM

LSC · 30104-31214 · 1111 bp

Back to Noronhia

Pi 0.0018

MarkerSeek score 60.20

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1111
variable_sites: 5
pi: 0.0018
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	ACTCGTAGTGTGGGGAAGAA	GCGACTGCACTATTCATTCT	50.0	45.0	58.0	55.9	1219-1232 bp	1.0	63.9
petN-psbM_p2	TCGTAGTGTGGGGAAGAAGT	GCGACTGCACTATTCATTCT	50.0	45.0	58.0	55.9	1217-1230 bp	1.0	63.9
petN-psbM_p3	CGTAGTGTGGGGAAGAAGTG	GCGACTGCACTATTCATTCT	55.0	45.0	57.9	55.9	1216-1229 bp	1.0	63.3
petN-psbM_p4	TAGTAAGTCTCGCTTGGGCT	GCGACTGCACTATTCATTCT	50.0	45.0	58.2	55.9	1271-1284 bp	1.0	62.7
petN-psbM_p5	TAGTGTGGGGAAGAAGTGGA	GCGACTGCACTATTCATTCT	50.0	45.0	57.6	55.9	1214-1227 bp	1.0	60.7