Hotspot

atpF-atpH

LSC · 14052-14413 · 362 bp

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Pi 0.0116

MarkerSeek score 64.10

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 362
variable_sites: 10
pi: 0.0116
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	TGGCCCAAAGAAACGAAAGA	CCGAGGCAGAGGGAAAAATA	45.0	50.0	57.9	57.3	515-521 bp	1.0	76.2
atpF-atpH_p2	GTGGCCCAAAGAAACGAAAG	CCGAGGCAGAGGGAAAAATA	50.0	50.0	57.9	57.3	516-522 bp	1.0	75.6
atpF-atpH_p3	AGTGGCCCAAAGAAACGAAA	CCGAGGCAGAGGGAAAAATA	45.0	50.0	58.2	57.3	517-523 bp	1.0	74.8
atpF-atpH_p4	TTAAATCCGAAACTCCCGGC	CCGAGGCAGAGGGAAAAATA	50.0	50.0	58.3	57.3	545-551 bp	1.0	74.4
atpF-atpH_p5	TGGCCCAAAGAAACGAAAGA	GCAGAGGGAAAAATACGAGGT	45.0	47.6	57.9	58.0	510-516 bp	1.0	73.8