Hotspot

psbI-trnS(GCU)

LSC · 9201-9328 · 128 bp

Back to Noronhia

Pi 0.0312

MarkerSeek score 72.00

Primer available yes

Label psbI-trnS(GCU)

Feature Metadata

label_name: psbI-trnS(GCU)
feature_type: igs
region: LSC
length_bp: 128
variable_sites: 6
pi: 0.0312
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 72.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-trnS(GCU)_p1	CGTAATCCTGGACGTGAAGA	TGGACTAAAGCGTCGGATTG	50.0	50.0	57.1	58.0	219-225 bp	1.0	76.8
psbI-trnS(GCU)_p2	CGTAATCCTGGACGTGAAGA	GTGGACTAAAGCGTCGGATT	50.0	50.0	57.1	58.0	220-226 bp	1.0	76.8
psbI-trnS(GCU)_p3	CGTAATCCTGGACGTGAAGA	ATTGGGAGAGATGGCTGAGT	50.0	50.0	57.1	58.1	238-244 bp	1.0	75.6
psbI-trnS(GCU)_p4	CGTAATCCTGGACGTGAAGAA	TGGACTAAAGCGTCGGATTG	47.6	50.0	57.8	58.0	219-225 bp	1.0	74.5
psbI-trnS(GCU)_p5	CGTAATCCTGGACGTGAAGAA	GTGGACTAAAGCGTCGGATT	47.6	50.0	57.8	58.0	220-226 bp	1.0	74.4