Hotspot

trnH(GUG)-psbA

LSC · 97-540 · 444 bp

Back to Noronhia

Pi 0.0149
MarkerSeek score 58.90
Primer available yes
Label trnH(GUG)-psbA

Feature Metadata

label_name
trnH(GUG)-psbA
feature_type
igs
region
LSC
length_bp
444
variable_sites
14
pi
0.0149
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
58.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH(GUG)-psbA_p1 TTAGTTATGGGCGAACGACG CCGTGCTAACCTTGGTATGG 50.0 55.0 58.1 58.3 623-628 bp 1.0 78.8
trnH(GUG)-psbA_p2 CTGCCTTAATCCACTTGGCT CCGTGCTAACCTTGGTATGG 50.0 55.0 57.9 58.3 568-573 bp 1.0 78.6
trnH(GUG)-psbA_p3 ACTGCCTTAATCCACTTGGC CCGTGCTAACCTTGGTATGG 50.0 55.0 58.2 58.3 569-574 bp 1.0 78.4
trnH(GUG)-psbA_p4 TTAGTTATGGGCGAACGACG ACCGTGCTAACCTTGGTATG 50.0 50.0 58.1 57.6 624-629 bp 1.0 78.3
trnH(GUG)-psbA_p5 CTGCCTTAATCCACTTGGCT ACCGTGCTAACCTTGGTATG 50.0 50.0 57.9 57.6 569-574 bp 1.0 78.1