Hotspot

ycf1

SSC · 107361-112370 · 5010 bp

Back to Nordenstamia

Pi 0.00273

MarkerSeek score 54.30

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5010
variable_sites: 20
pi: 0.00273
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TCGCCTCATATTGAATCCGA	TGATTCAAACGTCCAGTCCA	45.0	45.0	56.5	57.1	330-333 bp	1.0	62.2
ycf1_p2	TCGCCTCATATTGAATCCGAA	TGATTCAAACGTCCAGTCCA	42.9	45.0	57.3	57.1	330-333 bp	1.0	60.1
ycf1_p3	TCGCCTCATATTGAATCCGA	TTGATTCAAACGTCCAGTCCA	45.0	42.9	56.5	57.8	331-334 bp	1.0	59.9
ycf1_p4	TCGCCTCATATTGAATCCGA	TCAAACGTCCAGTCCATCTT	45.0	45.0	56.5	56.8	326-329 bp	1.0	59.8
ycf1_p5	TCGCCTCATATTGAATCCGA	TTCAAACGTCCAGTCCATCT	45.0	45.0	56.5	56.8	327-330 bp	1.0	59.8