Hotspot

rps11

LSC · 77912-78322 · 411 bp

Back to Nordenstamia

Pi 0.0308

MarkerSeek score 51.60

Primer available yes

Label rps11

Feature Metadata

label_name: rps11
feature_type: gene
region: LSC
length_bp: 411
variable_sites: 19
pi: 0.0308
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps11_p1	TTTCTCTTGTTTCTCTTGCT	GGGGGATCTGTTTTGACATGA	35.0	47.6	52.6	57.9	526 bp	1.0	42.9
rps11_p2	TTTCTCTTGTTTCTCTTGCT	CAGACTCGTTAAAGACCCGA	35.0	50.0	52.6	57.0	560 bp	1.0	42.7
rps11_p3	TTTCTCTTGTTTCTCTTGCT	TCAGACTCGTTAAAGACCCG	35.0	50.0	52.6	57.0	561 bp	1.0	42.7
rps11_p4	TCTCTTGTTTCTCTTGCTATTGA	CAGACTCGTTAAAGACCCGA	34.8	50.0	55.6	57.0	558 bp	1.0	42.7
rps11_p5	TCTCTTGTTTCTCTTGCTATTGA	TCAGACTCGTTAAAGACCCG	34.8	50.0	55.6	57.0	559 bp	1.0	42.7