Hotspot

petD

LSC · 75421-76628 · 1208 bp

Back to Nordenstamia

Pi 0.00166

MarkerSeek score 60.60

Primer available yes

Label petD

Feature Metadata

label_name: petD
feature_type: gene
region: LSC
length_bp: 1208
variable_sites: 3
pi: 0.00166
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD_p1	ATTTCCCTTGAACTCCGCAA	TGAAGCGACTGTTCCCTAGA	45.0	50.0	57.7	58.1	1334-1342 bp	1.0	77.7
petD_p2	TTTCCCTTGAACTCCGCAAT	TGAAGCGACTGTTCCCTAGA	45.0	50.0	57.7	58.1	1333-1341 bp	1.0	77.7
petD_p3	ATTTCCCTTGAACTCCGCAA	CTTTGAAGCGACTGTTCCCT	45.0	50.0	57.7	58.1	1337-1345 bp	1.0	77.4
petD_p4	TTTCCCTTGAACTCCGCAAT	CTTTGAAGCGACTGTTCCCT	45.0	50.0	57.7	58.1	1336-1344 bp	1.0	77.4
petD_p5	TTTCCCTTGAACTCCGCAAT	ACTTTGAAGCGACTGTTCCC	45.0	50.0	57.7	58.4	1337-1345 bp	1.0	75.1