Hotspot

ndhF

SSC · 123829-126054 · 2226 bp

Back to Nordenstamia

Pi 0.0015

MarkerSeek score 43.70

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2226
variable_sites: 5
pi: 0.0015
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 43.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	TGTCGCTTGGTCATATCATTTG	CTAGCACTGGGTAGACCTCA	40.9	55.0	57.1	57.9	2740-2773 bp	1.0	56.5
ndhF_p2	TGTCGCTTGGTCATATCATTTG	TCGTTGTGGTCGGACTCTAT	40.9	50.0	57.1	58.2	2928-2961 bp	1.0	56.3
ndhF_p3	TGTCGCTTGGTCATATCATTTG	ATCAATTCGGTCGTTGTGGT	40.9	45.0	57.1	57.8	2938-2971 bp	1.0	56.2
ndhF_p4	TGTCGCTTGGTCATATCATTTG	TCTCTTCCTTCTCCGAGCTC	40.9	55.0	57.1	58.2	2870-2903 bp	1.0	55.7
ndhF_p5	GCCCAAATTTTTATCCTTGCTCT	CTAGCACTGGGTAGACCTCA	39.1	55.0	57.9	57.9	2858-2891 bp	1.0	54.9