Hotspot

ycf15-rps12

IRa · 136148-137105 · 958 bp

Back to Nordenstamia

Pi 0.00278

MarkerSeek score 60.50

Primer available yes

Label ycf15-rps12

Feature Metadata

label_name: ycf15-rps12
feature_type: igs
region: IRa
length_bp: 958
variable_sites: 4
pi: 0.00278
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf15-rps12_p1	GAAAGGACTCACTAAGCCGG	TGGCAACTTTACGTAAGGCA	55.0	45.0	58.0	57.7	1033 bp	1.0	78.2
ycf15-rps12_p2	CACGGTACAATCCTCTTCCC	TGGCAACTTTACGTAAGGCA	55.0	45.0	57.7	57.7	1122 bp	1.0	75.7
ycf15-rps12_p3	GAAAGGACTCACTAAGCCGG	ACGTAAGGCAGAGTTTGGTT	55.0	45.0	58.0	57.4	1023 bp	1.0	75.2
ycf15-rps12_p4	TCACTAAGCCGGGATCACTA	TGGCAACTTTACGTAAGGCA	50.0	45.0	57.6	57.7	1025 bp	1.0	74.7
ycf15-rps12_p5	CTCGGTCCACAGAGACAAAA	TGGCAACTTTACGTAAGGCA	50.0	45.0	57.5	57.7	1085 bp	1.0	74.0