Hotspot

rpl32-ndhF

SSC · 122804-123828 · 1025 bp

Back to Nordenstamia

Pi 0.00752

MarkerSeek score 63.40

Primer available yes

Label rpl32-ndhF

Feature Metadata

label_name: rpl32-ndhF
feature_type: igs
region: SSC
length_bp: 1025
variable_sites: 11
pi: 0.00752
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 63.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-ndhF_p1	ACAAAGCTTTTAACGCTGCC	GTTGCCGTCGGAAAAAGAAG	45.0	50.0	57.9	58.0	1220-1266 bp	1.0	79.4
rpl32-ndhF_p2	AACGCTGCCCAATATCCTTT	GTTGCCGTCGGAAAAAGAAG	45.0	50.0	57.9	58.0	1209-1255 bp	1.0	79.4
rpl32-ndhF_p3	ACAAAGCTTTTAACGCTGCC	TGCCGTCGGAAAAAGAAGAA	45.0	45.0	57.9	58.0	1218-1264 bp	1.0	79.3
rpl32-ndhF_p4	ACAAAGCTTTTAACGCTGCC	TTGCCGTCGGAAAAAGAAGA	45.0	45.0	57.9	58.0	1219-1265 bp	1.0	79.3
rpl32-ndhF_p5	ACAAAGCTTTTAACGCTGCC	TACTTTTTGTTGCCGTCGGA	45.0	45.0	57.9	58.0	1228-1274 bp	1.0	79.3