Hotspot

rps12-ycf15

IRb · 96914-97871 · 958 bp

Back to Nordenstamia

Pi 0.00278

MarkerSeek score 60.50

Primer available yes

Label rps12-ycf15

Feature Metadata

label_name: rps12-ycf15
feature_type: igs
region: IRb
length_bp: 958
variable_sites: 4
pi: 0.00278
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps12-ycf15_p1	TGGCAACTTTACGTAAGGCA	GAAAGGACTCACTAAGCCGG	45.0	55.0	57.7	58.0	1033 bp	1.0	78.2
rps12-ycf15_p2	TGGCAACTTTACGTAAGGCA	CACGGTACAATCCTCTTCCC	45.0	55.0	57.7	57.7	1122 bp	1.0	75.7
rps12-ycf15_p3	ACGTAAGGCAGAGTTTGGTT	GAAAGGACTCACTAAGCCGG	45.0	55.0	57.4	58.0	1023 bp	1.0	75.2
rps12-ycf15_p4	TGGCAACTTTACGTAAGGCA	TCACTAAGCCGGGATCACTA	45.0	50.0	57.7	57.6	1025 bp	1.0	74.7
rps12-ycf15_p5	TGGCAACTTTACGTAAGGCA	CTCGGTCCACAGAGACAAAA	45.0	50.0	57.7	57.5	1085 bp	1.0	74.0