Hotspot

rps18-rpl20

LSC · 67199-67495 · 297 bp

Back to Nordenstamia

Pi 0.00676

MarkerSeek score 62.10

Primer available yes

Label rps18-rpl20

Feature Metadata

label_name: rps18-rpl20
feature_type: igs
region: LSC
length_bp: 297
variable_sites: 3
pi: 0.00676
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps18-rpl20_p1	AACCGAGTCGACCACTAGAA	TCTGTACAAGAGACAGTTGCT	50.0	42.9	58.1	56.6	445-458 bp	1.0	63.2
rps18-rpl20_p2	AAGAACCGAGTCGACCACTA	TCTGTACAAGAGACAGTTGCT	50.0	42.9	58.1	56.6	448-461 bp	1.0	63.1
rps18-rpl20_p3	TTGAAAGAACCGAGTCGACC	TCTGTACAAGAGACAGTTGCT	50.0	42.9	57.9	56.6	452-465 bp	1.0	62.7
rps18-rpl20_p4	AACCGAGTCGACCACTAGAA	TCTGTACAAGAGACAGTTGCTT	50.0	40.9	58.1	57.3	445-458 bp	1.0	60.8
rps18-rpl20_p5	AACCGAGTCGACCACTAGAA	TGTACAAGAGACAGTTGCTTCT	50.0	40.9	58.1	57.3	443-456 bp	1.0	60.8