Hotspot

accD-psaI

LSC · 58040-58720 · 681 bp

Back to Nordenstamia

Pi 0.00489

MarkerSeek score 61.70

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 681
variable_sites: 4
pi: 0.00489
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	TCCAGAAGGGCTTATTCGATC	AAAGAAGCCATTGCGATTGC	47.6	45.0	57.2	58.0	862 bp	1.0	66.3
accD-psaI_p2	CCAGAAGGGCTTATTCGATCT	AAAGAAGCCATTGCGATTGC	47.6	45.0	57.2	58.0	861 bp	1.0	66.3
accD-psaI_p3	TTCCAGAAGGGCTTATTCGA	AAAGAAGCCATTGCGATTGC	45.0	45.0	56.2	58.0	863 bp	1.0	66.3
accD-psaI_p4	TCCAGAAGGGCTTATTCGAT	AAAGAAGCCATTGCGATTGC	45.0	45.0	56.0	58.0	862 bp	1.0	64.4
accD-psaI_p5	TCCAGAAGGGCTTATTCGATC	TTGCGATTGCCGGAAATACT	47.6	45.0	57.2	58.3	852 bp	1.0	64.3