Hotspot

ndhC-trnV-TAC

LSC · 50071-50827 · 757 bp

Back to Nordenstamia

Pi 0.00352

MarkerSeek score 61.00

Primer available yes

Label ndhC-trnV-TAC

Feature Metadata

label_name: ndhC-trnV-TAC
feature_type: igs
region: LSC
length_bp: 757
variable_sites: 4
pi: 0.00352
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-TAC_p1	CCCTTTGCTAATCGGAGCTA	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.1	57.8	901 bp	1.0	71.5
ndhC-trnV-TAC_p2	TCGGAGCTAAAAATCCCGAA	AGAAGGTCTACGGTTCGAGT	45.0	50.0	56.9	57.8	890 bp	1.0	70.3
ndhC-trnV-TAC_p3	CCCTTTGCTAATCGGAGCTAA	AGAAGGTCTACGGTTCGAGT	47.6	50.0	57.8	57.8	901 bp	1.0	69.2
ndhC-trnV-TAC_p4	CCCTTTGCTAATCGGAGCTA	GAAGGTCTACGGTTCGAGTC	50.0	55.0	57.1	57.5	900 bp	1.0	68.7
ndhC-trnV-TAC_p5	GGCCCTTTGCTAATCGGAG	AGAAGGTCTACGGTTCGAGT	57.9	50.0	58.3	57.8	903 bp	1.0	68.4