Hotspot

trnT-GGT-psbD

LSC · 31049-32174 · 1126 bp

Back to Nordenstamia

Pi 0.0042
MarkerSeek score 62.20
Primer available yes
Label trnT-GGT-psbD

Feature Metadata

label_name
trnT-GGT-psbD
feature_type
igs
region
LSC
length_bp
1126
variable_sites
7
pi
0.0042
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
62.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGT-psbD_p1 TACAGTGACCCGTCAGGAAT GAAACGGTCCCTACGTAACC 50.0 55.0 58.1 58.0 1307-1321 bp 1.0 80.7
trnT-GGT-psbD_p2 TAACTCAGCGGTAGAGCACT GAAACGGTCCCTACGTAACC 50.0 55.0 58.2 58.0 1261-1275 bp 1.0 80.0
trnT-GGT-psbD_p3 TAGTACAGTGACCCGTCAGG GAAACGGTCCCTACGTAACC 55.0 55.0 58.2 58.0 1310-1324 bp 1.0 79.9
trnT-GGT-psbD_p4 TACAGTGACCCGTCAGGAAT GGACCAGCCTACAAAAACGA 50.0 50.0 58.1 58.1 1325-1339 bp 1.0 79.8
trnT-GGT-psbD_p5 GTGACCCGTCAGGAATTCAA GAAACGGTCCCTACGTAACC 50.0 55.0 57.8 58.0 1303-1317 bp 1.0 79.8