Hotspot

trnK-TTT-rps16

LSC · 4409-5248 · 840 bp

Back to Nordenstamia

Pi 0.00238
MarkerSeek score 60.30
Primer available yes
Label trnK-TTT-rps16

Feature Metadata

label_name
trnK-TTT-rps16
feature_type
igs
region
LSC
length_bp
840
variable_sites
3
pi
0.00238
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-TTT-rps16_p1 AAAGCCGAGTACTCTACCGT TTCCTTGAAAAAGGCGCTCA 50.0 45.0 58.2 58.3 976 bp 1.0 76.4
trnK-TTT-rps16_p2 AAGCCGAGTACTCTACCGTT TTCCTTGAAAAAGGCGCTCA 50.0 45.0 58.2 58.3 975 bp 1.0 76.4
trnK-TTT-rps16_p3 TCAGTCGTGGTCTTCCAAAC TTCCTTGAAAAAGGCGCTCA 50.0 45.0 57.8 58.3 1061 bp 1.0 75.8
trnK-TTT-rps16_p4 CAGTCGTGGTCTTCCAAACT TTCCTTGAAAAAGGCGCTCA 50.0 45.0 57.8 58.3 1060 bp 1.0 75.8
trnK-TTT-rps16_p5 TCCTAGCCGCACTTAAAAGC TTCCTTGAAAAAGGCGCTCA 50.0 45.0 58.3 58.3 991 bp 1.0 75.6