Hotspot

trnW-CCA

LSC · 67671-67744 · 74 bp

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Pi 0.0135
MarkerSeek score 60.30
Primer available yes
Label trnW-CCA

Feature Metadata

label_name
trnW-CCA
feature_type
tRNA
region
LSC
length_bp
74
variable_sites
1
pi
0.0135
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnW-CCA_p1 GATTGACCTCCTCCTTGCAG ACGCATTGGGGGTACAAAAA 55.0 45.0 58.0 58.3 238 bp 1.0 77.7
trnW-CCA_p2 GATTGACCTCCTCCTTGCAG TATACAAGACGCATTGGGGG 55.0 50.0 58.0 57.4 246 bp 1.0 75.0
trnW-CCA_p3 GATTGACCTCCTCCTTGCAG TACAAGACGCATTGGGGGTA 55.0 50.0 58.0 58.7 244 bp 1.0 74.3
trnW-CCA_p4 GATTGACCTCCTCCTTGCAG GACGCATTGGGGGTACAAAA 55.0 50.0 58.0 58.8 239 bp 1.0 74.0
trnW-CCA_p5 TTGATTGACCTCCTCCTTGC ACGCATTGGGGGTACAAAAA 50.0 45.0 57.5 58.3 240 bp 1.0 74.0