Hotspot

rps3

LSC · 84171-84833 · 663 bp

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Pi 0.00151
MarkerSeek score 49.20
Primer available yes
Label rps3

Feature Metadata

label_name
rps3
feature_type
gene
region
LSC
length_bp
663
variable_sites
1
pi
0.00151
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
49.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps3_p1 AACGGAGCTCCCAACAAAAT TCTGACTTTCGCGAGACATG 45.0 50.0 58.0 58.0 1866-1874 bp 1.0 80.2
rps3_p2 ACGGAGCTCCCAACAAAATT TCTGACTTTCGCGAGACATG 45.0 50.0 58.0 58.0 1865-1873 bp 1.0 80.2
rps3_p3 AACGGAGCTCCCAACAAAAT CGGGCGTTCTTATGAGGAAA 45.0 50.0 58.0 58.0 1666-1674 bp 1.0 80.2
rps3_p4 ACGGAGCTCCCAACAAAATT CGGGCGTTCTTATGAGGAAA 45.0 50.0 58.0 58.0 1665-1673 bp 1.0 80.2
rps3_p5 ACGGAGCTCCCAACAAAATT ATGTCTGTTTTCAAAGCGCG 45.0 45.0 58.0 58.0 1706-1714 bp 1.0 80.1