Hotspot

ndhD

SSC · 117398-118900 · 1503 bp

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Pi 0.00266
MarkerSeek score 54.90
Primer available yes
Label ndhD

Feature Metadata

label_name
ndhD
feature_type
gene
region
SSC
length_bp
1503
variable_sites
4
pi
0.00266
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
54.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhD_p1 TGTGTTGTACAGCTCAAAAATCT ACCGACAAAAACCCGTACTC 34.8 50.0 56.9 58.1 1651 bp 1.0 46.5
ndhD_p2 TGTGTTGTACAGCTCAAAAATCT CGACAAAAACCCGTACTCGA 34.8 50.0 56.9 58.2 1649 bp 1.0 45.7
ndhD_p3 TGTGTTGTACAGCTCAAAAA ACCGACAAAAACCCGTACTC 35.0 50.0 53.6 58.1 1651 bp 1.0 43.8
ndhD_p4 TTGTGTTGTACAGCTCAAAA ACCGACAAAAACCCGTACTC 35.0 50.0 53.6 58.1 1652 bp 1.0 43.8
ndhD_p5 TTTGTGTTGTACAGCTCAAA ACCGACAAAAACCCGTACTC 35.0 50.0 53.6 58.1 1653 bp 1.0 43.8