Hotspot

atpF

LSC · 12434-13820 · 1387 bp

Pi 0.00216

MarkerSeek score 52.00

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: LSC
length_bp: 1387
variable_sites: 3
pi: 0.00216
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	ACTAATTTCGTCAGCTCGAA	AAAAAGGGCGAGTCAAGTGA	40.0	45.0	54.9	57.7	1567 bp	1.0	52.2
atpF_p2	ACTAATTTCGTCAGCTCGAA	GGGCGAGTCAAGTGATACAA	40.0	50.0	54.9	57.6	1562 bp	1.0	51.9
atpF_p3	ACTAATTTCGTCAGCTCGAA	AGGGCGAGTCAAGTGATACA	40.0	50.0	54.9	58.4	1563 bp	1.0	51.4
atpF_p4	ACTAATTTCGTCAGCTCGAA	AAAAGGGCGAGTCAAGTGAT	40.0	45.0	54.9	57.4	1566 bp	1.0	50.4
atpF_p5	ACTAATTTCGTCAGCTCGAA	AAGGGCGAGTCAAGTGATAC	40.0	50.0	54.9	57.3	1564 bp	1.0	49.6