Hotspot

ccsA-ndhD

SSC · 117175-117397 · 223 bp

Back to Nolina

Pi 0.0179
MarkerSeek score 62.80
Primer available yes
Label ccsA-ndhD

Feature Metadata

label_name
ccsA-ndhD
feature_type
igs
region
SSC
length_bp
223
variable_sites
4
pi
0.0179
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA-ndhD_p1 ATTCGTCACTTGTAGCGTCC TCGCTATCAGTTGACAAGGT 50.0 45.0 58.0 56.6 393-396 bp 1.0 69.4
ccsA-ndhD_p2 ATTCGTCACTTGTAGCGTCC TTCGCTATCAGTTGACAAGGT 50.0 42.9 58.0 57.3 394-397 bp 1.0 67.3
ccsA-ndhD_p3 ATTCGTCACTTGTAGCGTCC TCAGTTGACAAGGTACAAGCT 50.0 42.9 58.0 57.2 387-390 bp 1.0 66.1
ccsA-ndhD_p4 CGTCACTTGTAGCGTCCATA TCGCTATCAGTTGACAAGGT 50.0 45.0 57.4 56.6 390-393 bp 1.0 65.0
ccsA-ndhD_p5 ATTCGTCACTTGTAGCGTCC TTTCGCTATCAGTTGACAAGGT 50.0 40.9 58.0 58.0 395-398 bp 1.0 64.7