Hotspot

rpl32-trnL-UAG

SSC · 115122-116044 · 923 bp

Back to Nolina

Pi 0.00217
MarkerSeek score 54.00
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
923
variable_sites
2
pi
0.00217
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
54.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 TGTGCGACAAACAAATAAGA AGCGTGTCTACCGATTTCAC 35.0 50.0 53.8 58.0 986 bp 1.0 46.3
rpl32-trnL-UAG_p2 TGTGCGACAAACAAATAAGA CAGCGTGTCTACCGATTTCA 35.0 50.0 53.8 58.0 987 bp 1.0 46.3
rpl32-trnL-UAG_p3 TGTGCGACAAACAAATAAGA TTTACAGTATGCCGCCACTC 35.0 50.0 53.8 58.0 1046 bp 1.0 46.2
rpl32-trnL-UAG_p4 TGTGCGACAAACAAATAAGA TCCTAAGAGCAGCGTGTCTA 35.0 50.0 53.8 57.9 996 bp 1.0 45.4
rpl32-trnL-UAG_p5 TGTGCGACAAACAAATAAGAA AGCGTGTCTACCGATTTCAC 33.3 50.0 54.6 58.0 986 bp 1.0 45.2